The MAGUS Lum 400 fluorescence microscope is designed for diagnostics and research and can be used for fluorescence (reflected light) and brightfield (transmitted light) microscopy methods. With additional instruments it is possible to use polarization, dark field and phase contrast techniques. Areas of application of the fluorescence microscope: DNA analysis, study of pathogens, health and epidemiological surveillance, forensic assessment, etc.
Optics
It is a Trinocular microscope: The binocular head is inclined 30°, and the digital camera (not included) is installed on the trinocular tube. The eyepiece tubes are rotatable 360° so it is easy to adjust them to suit the user's height.
You can install five objectives in the nosepiece. Four infinity plan achromatic objectives are included. One of them is fluorescent with 40x magnification and is designed for fluorescence observations. 4x, 10x and 100x objectives are used for the bright field microscopy technique. In the available slot of the nosepiece it is possible to mount an additional objective to achieve greater magnification within the magnification range of the microscope.
The nosepiece is oriented towards the inside of the instrument, so that the user can see which objective is inserted in the optical path.
Illumination
The reflected light source is a 100 W mercury vapor lamp It is located in the lamp housing which offers efficient heat dissipation: the lamp does not overheat even after long hours of operation. It is possible to center the lamp on three axes to provide optimal illumination of the work area. If you need to replace it, simply remove and install it. The mercury vapor lamp is suitable for fluorescent pigments because they have distinct peaks. The reflected light illuminator contains four excitation filters: ultraviolet (UV), violet (V), blue (B) and green (G).
Transmitted light light source: 30 W halogen lamp It has enough intensity to work with any lens, regardless of magnification. Thanks to the color temperature of the illumination, even long-term observations do not tire the eyes. The lighting system also includes a capacitor. There is a slot for a slider (contrast phase or dark field).
In transmitted and reflected light, the Köhler illumination method can be set. With this illumination method, the edges are not darkened, the sample is clearly visible, and the image has no artifacts.
Stage and focusing mechanism
The microscope is equipped with an ergonomic stage without a positioning rack. For reflected light observations, it is necessary to place a black plate on the stage, in order to reduce any unwanted light. The sample holder plate is removable, if necessary, by loosening two screws. The focus knobs are coaxial and are located on both sides of the microscope. The coarse focus mechanism is equipped with a fixing knob and tension adjustment and the fine focus is used for sharpness adjustment.
Accessories
The MAGUS Lum 400 microscope can be equipped with additional accessories: digital camera, phase contrast device, polarization device, dark field condenser, eyepieces and objectives, phase contrast and dark field slider. If you want to measure objects, you can equip the microscope with a calibration slide.
Key features:
- Microscopy techniques: fluorescence in reflected light, bright field in transmitted light
- Other research methods with optional accessories: phase contrast, simple polarization, dark field
- Trinocular head with trinocular tube for the installation of a digital camera; 360° rotatable eyepiece tubes
- Infinity plan achromatic objectives, including a fluorescent objective
- Reflected light source: 100 W mercury vapor lamp, transmitted light source: lamp 30 W halogen
- Köhler illumination method in transmitted and reflected light
- Fluorescence filters: ultraviolet (UV), violet (V), blue (B), green (G)
- Ergonomic stage with a removable sample holder and without positioning rack
The package contains:
| Brand | MAGUS |
| Warranty | 5 лет |
| EAN | 5905555018140 |
| Package size (WxDxH) | 45x30x96 cm |
| Shipping weight | 16.5 kg |
| Type | biological, light/optical |
| Head | trinocular |
| Vertical tube | Gemel head (Siedentopf type, with 360° rotation) |
| Head inclination angle | 30° |
| Magnification, x | 40–1000 in basic configuration (*optional: 40–1250/1500/2000/2500) |
| Magnification range | 800x to 1280x |
| Eyepiece tube diameter, mm | 30 |
| Eyepieces | 10х/22 mm, eye relief: 10 mm (*optional: 10x/22 mm with graduated scale, 12.5x/14; 15x/15; 20x/12; 25x/9) |
| Objectives | infinity planachromatic and fluorescent objectives: PL 4x/0.10, PL 10x/0.25, PL FL 40x/0.85, PL 100x/1.25 (oil); parfocal distance 45 mm (*optional: PL FL 10x/0.35, PL 20х/0.40, PL 60x/0.80 ∞/0.17) |
| Revolver nosepiece | for 5 objectives |
| Working distance, mm | 19.8 (4x); 5.0 (10x); 0.42 (FL 40x); 0.36 (100x); 2.37 (FL 10х); ); 8.8 (20х); 0.46 (60х) |
| Interpupillary distance, mm | 48 — 75 |
| Stage, mm | 180x150 |
| Range of movement of the stage, mm | 75/50 |
| Stage characteristics | two-axis mechanical translator, without positioning rack |
| Condenser | condenser from Abbe, NA 1.25, adjustable centering, adjustable height, adjustable aperture diaphragm, with slot for dark field slider and phase contrast slider, dovetail mount |
| Diaphragm | adjustable aperture diaphragm, field diaphragm with adjustable iris |
| Focus | coaxial, coarse focus (21 mm, 39 .8 mm/turn, with fixing knob and micrometer screw tension adjustment knob) and fine focus (0.002 mm micrometer screw) |
| Illumination | halogen, fluorescent |
| Brightness adjustment | yes |
| Power supply | AC mains, 85–265 V, 50/60 Hz |
| Type of light source | reflected light: mercury vapor lamp 100W; transmitted light: 12 V halogen lamp, 30 W |
| Optical filters | yes |
| Operating temperature range, °C | 5 — 35 |
| Possibility of connecting additional equipment | phase contrast devices (condenser and objectives), dark field condenser (dry or oil immersion), polarization instruments (polarizer and analyzer), dark field slider |
| User level | expert users, professionals |
| Assembly and installation level of difficulty | complicated |
| Fluorescent module | filters: ultraviolet (UV), violet (V), blue (B), green (G) |
| Fluorescence filter: type of filter/excitation wavelength/dichroic mirror/emission wavelength | ultraviolet (UV), 320–380 nm/425 nm/435 nm; violet (V) 380–415 nm/455 nm/475 nm; blue (B) 450–490 nm/505 nm/515 nm; green (G) 495–555 nm/585 nm/595 nm |
| Application | laboratory/medical |
| Illumination position | double |
| Research method | bright field, fluorescence |
| Set with pencil case/case/bag | dust cover |
