The MAGUS Lum V500L is an inverted microscope for fluorescence microscopy in reflected light and brightfield and phase contrast microscopy in transmitted light. For fluorescence microscopy, filters for DAPI, FITC and TRITC fluorochromes are used. The inverted design allows you to analyze samples contained in laboratory glassware, such as Petri dishes, test tubes, etc. The height of the glassware can reach 55 mm or 165 mm when the stand is tilted. The optics are designed for laboratory glassware with a 1.2 mm thick bottom. This microscope is ideal for research and routine work in the laboratory and can be used for teaching purposes.
Digital camera
The digital camera MAGUS CLM90 is designed for the dark field and fluorescence microscopy technique.
The camera is equipped with a 7.1 MP sensor and produces realistic images at a resolution of 3200x2200 px. It is recommended to use the camera with 4x, 10x, 20x and 40x lenses. When using low magnification lenses, the camera allows the user to see sharper details.
Videos are recorded at 51.3 fps or 133.8 fps depending on the resolution. Videos flow smoothly with smooth, subtle transitions between frames. The movement of the sample is shown in real time, without delays. The camera facilitates the observation of moving objects and is suitable for academic demonstrations.
The camera is equipped with a USB 3.0 interface. The data transfer speed is 10 times faster than USB 2.0 cameras. The high-speed camera is recommended for professional laboratories, research or university education.
Optics
Two options for installing a digital camera and a Monitor: The trinocular tube is designed to accommodate a monitor (not included), while the side port on the microscope frame is designed for a digital camera (not included). On both optical paths there is a beam splitter: 100/0 and 0/100 (microscope frame), 80/20 (trinocular head). The 45° inclination of the eyepiece tube allows you to make observations in complete comfort. When assembling the microscope, the user can rotate the optical tubes 180°.
The package includes six planachromatic objectives: three for use in bright field and fluorescence microscopy, the others three for phase contrast microscopy. The lenses are infinitely corrected and have a long working distance. The nosepiece revolver has six slots for housing as many objectives. The nosepiece is placed on a support underneath the stage.
Illumination
The fluorescence excitation source is a 5 W LED with a life of 50,000 hours, without the need for replacement during the useful life of the instrument. The instrument is equipped with three excitation filters: filters for DAPI, FITC and TRITC fluorochromes. LED lighting is more energy efficient and easier to maintain than a mercury vapor lamp, making it advisable for use in university-level education. LED lighting is consistent in terms of color temperature. The LED turns on and off quickly and does not overheat during prolonged use.
The light source for transmitted light observations is always a 5 W LED. Its light intensity is sufficient for each of the objectives used for bright field or phase contrast microscopy techniques.
Both light sources, both reflected and transmitted light, can be adjusted according to the Köhler method. This allows you to reach the maximum resolution limit for each objective lens and obtain a clear, sharp image without artefacts or edge darkening.
The microscope is equipped with a phase contrast condenser: three slots are used for phase contrast objectives with 10x, 20x and 40x magnification, while the fourth slot is for bright field microscopy. Phase contrast rings can be centered. You can switch from one microscopy technique to another just by turning a knob.
Stage and focusing mechanism
The fixed stage is equipped with a translator insert for moving laboratory glassware and is equipped with three supports for plates of various sizes. Laboratory glassware can be moved on the horizontal plane, along two axes perpendicular to each other. The microscope features a mechanism for coarse and fine focusing. The coarse focus mechanism is equipped with a fixing knob and the tension of the coarse screw can be adjusted. The knobs have an ergonomic design that allows the user to maintain a relaxed posture while working.
Accessories
The microscope can be equipped with additional eyepieces , calibration slides or a digital camera from the MAGUS line of accessories.
Key features of the microscope:
- Inverted design for observing samples contained in laboratory glassware with a bottom thickness of 1.2 mm and a height of up to 55 mm, or 165 mm with an inclined support
- Microscopy techniques: fluorescence (reflected light), bright field and contrast phase (transmitted light)
- Köhler illumination configurable for transmitted and reflected light; light source with long-life 5 W LED, without the need to replace bulbs
- The instrument is equipped with three excitation filters: filters for DAPI, FITC and TRITC fluorochromes
- Phase contrast condenser, allows for quick and practical switching between the various microscopy techniques
- Trinocular head; two options for installing a camera or monitor: beam splitter with ratio 100/0 and 0/100 (microscope frame), 80/20 (trinocular head)
Key camera features:
- For darkfield and fluorescence microscopy with 4x, 10x, 20x or 40x objectives
- 7.1 MP resolution: when making observations with low magnification lenses, the camera will allow the user to see sharper details
- 51.3 fps and 133.8 fps, respectively at 3200x2200 pixels and 1584x1100 pixel resolution, for l observation of objects with moving elements, for video recording and for moving the specimen without jerks or delays
- The Peltier cell lowers the temperature of the sensor during prolonged operation and eliminates thermal noise, allowing so you can use a long shutter speed when taking photographs
- Global shutter for fast signal reading by the sensor, greater image brightness without optical effects when observing moving objects
- The monochrome back-illuminated SONY Exmor CMOS sensor offers images with low noise and high light sensitivity even in low light conditions. You can get sharper, brighter and more saturated color images
- USB 3.0 interface for fast and smooth data transfer
- Software for recording and editing photos and videos, display functions external, linear and angular measurements
The package contains:
- MAGUS CLM90 digital camera (digital camera, USB cable, adapter 12 V, 3 A, case, installation CD with drivers and software, user manual and warranty card)
- Base with integrated power input, light source for reflected and transmitted light observations, condenser, mechanism focusing mechanism, stage and nosepiece
- Trinocular head
- Infinity planachromatic objective: PLL 10x/0.25 for phase contrast, WD 4.3 mm
- Plan achromatic infinity objective: PLL 20х/0.40 WD 8.0 mm
- Plan achromatic infinity objective: PLL 40x/0.60 WD: 3.5 mm
- Plan achromatic infinity objective: PLL 10x/0.25 PHP2 WD 4.3 mm
- Plan achromatic infinity objective: PLL 20x/0.40 PHP2 WD 8.0 mm
- Infinity planachromatic objective: PLL 40x/0.60 PHP2 WD 3.5 mm
- 10x/22 mm eyepiece with long eye relief (2 pcs.)
- Screen UV
- 1x C-mount adapter
- Allen key
- Power cable
- Dust cover
- Manual user and warranty certificate
Available on request:
- 10x/22 mm eyepiece with graduated scale
- Eyepiece 12.5x/14 mm (2 pcs.)
- Eyepiece 15x/15 mm (2 pcs.)
- Eyepiece 20x/12 mm (2 pcs.) li>
- Eyepiece 25x/9 mm (2 pcs.)
- Calibration glass
| Brand | MAGUS |
| Warranty | 5 лет |
| EAN | 5905555018249 |
| Package size (WxDxH) | 38x61x82 cm |
| Shipping weight |
| Delimiter | MAGUS Lum V500L inverted fluorescence microscope |
| Type | biological, light/optical |
| Head | trinocular |
| Vertical tube< /td> | Siedentopf, 180° rotatable |
| Magnification, x | 100–400 in basic configuration (*optional: 40–500/ 600/800/1000) |
| Eyepiece tube diameter, mm | 30 |
| Eyepieces |
| td> | 10х/22 mm, eye relief: 10 mm (*optional: 10x/22 mm with graduated scale, 12.5x/14; 15x/15; 20x/12; 25x/9) |
| Objectives | plan achromatic objectives at infinity (∞): PLL 10x/0.25/4.3; PLL 20x/0.40/8.0; PLL 40x/0.60/3.5; phase contrast: PLL 10x/0.25/4.3 PHP2; PLL 20x/0.40/8.0 PHP2; PLL 40x/0.60/3.5 PHP2; parfocal distance: 45 mm |
| Nosepiece revolver | for 6 objectives |
| Working distance, mm | 4.3 (10х); 8.0 (20x); 3.5 (40x) |
| Interpupillary distance, mm | 48 — 75 |
| Stage, mm | 227x208 |
| Range of movement of the stage, mm | 77/134.5 |
| Table features | fixed, with translator insert and Ø118 mm glass plates; Ø118 mm; plate supports: 86x129.5 mm, Ø87.5 mm; 34x77.5 mm, Ø68.5 mm; 57x82 mm, Ø60 mm |
| Capacitor | NA 0.6; working distance: 55 mm; phase contrast turret; with fixing screws |
| Diaphragm | iris aperture diaphragm |
| Focus | coaxial knobs for coarse and fine focusing (macro and fine), positioned on both sides |
| Illumination | halogen, fluorescent< /td> |
| Brightness adjustment | yes |
| Power supply | AC mains, 85 –265 V, 50/60 Hz |
| Type of light source | light source for reflected light observations: 5 W LED; Light source for transmitted light observations: 5 W LED |
| Optical filters | yes |
| Operating temperature range, °C | 5 — 35 |
| Special features | phase contrast condenser (turret) with available housing and phase contrast ring plates for 10x, 20x, and 40x objectives; auxiliary centering microscope |
| User level | expert users, professionals |
| Level of difficulty assembly and installation | complicated |
| Fluorescent module | filters: ultraviolet (UV), violet (V), blue (B), green (G) |
| Fluorescence filter: filter type/excitation wavelength/dichroic mirror/emission wavelength | ultraviolet ( UV), 320–380 nm/425 nm/435 nm; violet (V) 380–415 nm/455 nm/475 nm; blue (B) 450–490 nm/505 nm/515 nm; green (G) 495–555 nm/585 nm/595 nm |
| Application | laboratory/medical |
| Illumination position | double |
| Research method | bright field, phase contrast microscope, fluorescence |
| Set with pencil case/case/bag | dust cover |
| === Delimiter ===< /td> | Digital camera |
| Sensor | SONY Exmor CMOS |
| Color/ monochrome | monochrome |
| Megapixel | 7.1 |
| Maximum resolution, px | 3200x2200 |
| Sensor size | 1.1" (14.4x9.9 mm) |
| Pixel size, µm | 4.5x4.5 |
| Two-phase thermoelectric module (Peltier cell) to set the temperature at 42°C below room temperature | yes |
| Sensitivity to light | 3354 mV at 1/30 s< /td> |
| Signal-to-noise ratio | 0.15 mV at 1/30 s |
| Exposure time | 0.1 ms – 1 h |
| Video recording | yes |
| 51.3@3200x2200, 133.8@1584x1100 |
| ADC digital capacity (bit) | 8/12 (selectable) |
| Image format | *.jpg, *.bmp, *.png, *.tif td> |
| Video format | recording: *.wmv, *.avi, *.h264 (Windows 8 and later), *h265 (Windows 10 and later)< /td> |
| Spectral range, nm | 380–650 (with IR filter and anti-reflection filter) |
| Type shutter speed | Global shutter |
| White balance | manual, automatic |
| manual, automatic |
| Software features | image size, brightness, exposure control |
| Software | MAGUS View |
| Output | USB 3.0, 5 Gb/s< /td> |
| System requirements | Windows 8/10/11 (32-bit and 64-bit), Mac OS Core 2 or later, minimum 2 GB RAM, USB 3.0 port, CD-ROM, 17" or larger |
| Mount type | C-mount |
| Body | CNC aluminum alloy |
| Camera power supply | DC, 5 V, from computer USB port; 12 V, 3 A adapter for the Peltier cell |
| Camera operating temperature range, °C | -10 — 50 |
| Humidity operating range, % | 30 — 80 |
