The MAGUS Lum V500 inverted fluorescence microscope is designed for observing samples in laboratory glassware with a height of up to 55 mm and a bottom thickness of up to 1.2 mm. By tilting the illuminator support, it becomes possible to use larger laboratory glassware, up to 165 mm. Samples can be observed in reflected light (fluorescence microscopy technique) and in transmitted light (with bright field and phase contrast microscopy). The main fields of application of this microscope are: medicine, pharmacology, biochemistry, epidemiology, etc.
Optics
This microscope is equipped with a trinocular head with vertical optical tube. There is a side camera port on the microscope frame. This design choice allows you to simultaneously install a digital camera and a monitor (not included) on the microscope. The beam splitter of the trinocular head has a ratio of 80/20, while that of the microscope frame is 100/0 and 0/100. The eyepiece tubes are rotatable through 180°, which allows the eye relief to be adjusted to suit the user.
The nosepiece is placed on a stand underneath the stage. It is equipped with six free slots, for housing as many lenses. The package includes three objectives for phase contrast and three objectives for fluorescence and brightfield observations. These are planachromatic objectives with a long working distance, designed for laboratory glassware with a 1.2 mm thick bottom.
Illumination
The source of Reflected light is a 100W mercury vapor lamp. It is placed in a lamp holder for better heat dissipation and less risk of overheating during extended use. This lamp emits light in a wide range of wavelengths with various peak intensities, making it perfect for working with a variety of fluorophores. Fluorescence filters: ultraviolet (UV), violet (V), blue (B), and green (G). The mercury vapor lamp illuminates the sample intensely, providing good visibility and can be easily replaced when necessary.
The transmitted light is provided by a 30 W halogen lamp. The illumination provided is intense and with a warm color temperature. As a result, observation through each of the lenses is always efficient and comfortable for the eyes. The microscope has a phase contrast condenser with four slots: one is used for brightfield observations and the other three are designed for 10x, 20x and 40x phase contrast objectives. Phase contrast rings can be centered. To switch from bright field observation to phase contrast techniques, and vice versa, simply rotate the disk. This is particularly practical for professional researchers, as it saves time.
Application of Köhler illumination for transmitted and reflected light observations. Using this method allows you to obtain clear and sharp images, in high resolution, without artifacts or darkening of the edges.
Focusing stage and mechanism
The stage is fixed and is equipped with a mechanism for moving the laboratory glassware on which the sample is positioned. Laboratory glassware can be moved along the horizontal plane, along two axes perpendicular to each other. The movement is smooth and precise and the mechanism includes four holders for accommodating glassware of various sizes. The sharpness of the microscope image is adjusted by rotating the coarse and fine focus knobs (macro and fine screw). The coarse focus is equipped with a fixing knob and the tension of the coarse screw can be adjusted. The focus knobs are coaxial and are located on both sides of the frame.
Accessories
This product line includes additional accessories for the microscope MAGUS Lum V500: eyepieces, digital cameras and calibration slides.
Key features:
- Reflected light fluorescence microscopy and field microscopy clear and phase contrast in transmitted light
- Possibility of observing samples inside laboratory glassware with a height of up to 55 mm, or 165 mm with inclined support
- vapor lamp 100 W mercury lamp for reflected light observations, 30 W halogen lamp for transmitted light observations, configurable Köhler illumination
- Four fluorescence filters: ultraviolet (UV), violet (V), blue ( B) and green (G)
- Trinocular head rotatable at 180°. Two options for installing a digital camera and monitor: 80/20 beam splitter (trinocular head) or 100/0 or 0/100 beam splitter (microscope frame)
- Stage with slots for Petri dishes and laboratory glassware of various diameters
- Phase contrast condenser with bright field aperture and three phase contrast apertures
The package contains:
- Stand with light source for transmitted light observations and condenser, focusing mechanism, stage and nosepiece
- Fluorescence unit
- Mercury vapor lamp housing
- Trinocular head
- Planachromatic infinity objective: PLL 10x/0.25 for phase contrast, WD 4.3 mm
- Plan achromatic infinity objective: PLL 20х/0.40 WD 8.0 mm
- Plan achromatic infinity objective: PLL 40х/0.60 WD 3.5 mm
- Infinity plan achromatic objective: PLL 10x/0.25 PHP2 WD 4.3 mm
- Infinity plan achromatic objective: PLL 20x/0.40 PHP2 WD 8.0 mm
- Infinity planachromatic objective: PLL 40x/0.60 PHP2 WD 3.5 mm
- 10x/22 mm eyepiece with long eye relief (2 pcs.)
- UV screen
- 1x C-mount adapter
- Allen key
- Power supply for the mercury vapor lamp
- Power cable
- Reflected light illuminator power cable
- Dust cover
- User manual and warranty certificate
Available on request:
- 10x/22 mm eyepiece with graduated scale
- 12.5x/14 mm eyepiece (2 pcs.)
- 15x eyepiece /15 mm (2 pcs.)
- Eyepiece 20x/12 mm (2 pcs.)
- Eyepiece 25x/9 mm (2 pcs.)
- Camera digital
- Calibration glass
| Brand | MAGUS |
| Warranty | 5 лет |
| EAN | 5905555018201 |
| Package size (WxDxH) | 38x61x82 cm |
| Shipping weight | 26 kg |
| Type | biological, light/optical |
| Tested | trinocular |
| Vertical tube | Siedentopf, 180° rotatable |
| Magnification, x | 100–400 in basic configuration ( *optional: 40–500/600/800/1000) |
| Eyepiece tube diameter, mm | 30 |
| Eyepieces | 10х/22 mm, eye relief: 10 mm (*optional: 10x/22 mm with graduated scale, 12.5x/14; 15x/15; 20x/12; 25x/9) |
| Objectives | plan achromatic objectives at infinity (∞): PLL 10x/0.25/4.3; PLL 20x/0.40/8.0; PLL 40x/0.60/3.5; phase contrast: PLL 10x/0.25/4.3 PHP2; PLL 20x/0.40/8.0 PHP2; PLL 40x/0.60/3.5 PHP2; parfocal distance: 45 mm |
| Nosepiece revolver | for 6 objectives |
| Working distance, mm | 4.3 (10х); 8.0 (20x); 3.5 (40x) |
| Interpupillary distance, mm | 48 — 75 |
| Stage, mm | 227x208 |
| Range of movement of the stage, mm | 77/134.5 |
| Table features | fixed, with Ø118 mm glass plate and mechanical slider; plate holder: 86x129.5, Ø90 mm; 34x77.5 mm, Ø68.5 mm; 57x82 mm, Ø60 mm; 29x77.5 mm, Ø35 mm |
| Capacitor | NA 0.6; working distance: 55 mm; phase contrast turret; with fixing screws |
| Diaphragm | adjustable aperture diaphragm, field diaphragm with adjustable iris |
| Focus | coaxial, coarse focus (with micrometer screw tension adjustment knob and fixing knob) and fine focus (0.002 mm micrometer screw) |
| Lighting | halogen, fluorescent |
| Brightness adjustment | yes |
| Power supply | AC mains, 85–265 V, 50/60 Hz |
| Type of light source | reflected light: 100 W mercury vapor lamp; transmitted light: 12 V halogen lamp, 30 W |
| Optical filters | yes |
| Operating temperature range, °C | 5 — 35 |
| Special features | phase contrast condenser (turret) with available housing and phase contrast ring plates for 10x, 20x, and 40x objectives; auxiliary centering microscope |
| User level | expert users, professionals |
| Level of difficulty assembly and installation | complicated |
| Fluorescent module | filters: ultraviolet (UV), violet (V), blue (B), green (G) |
| Fluorescence filter: filter type/excitation wavelength/dichroic mirror/emission wavelength | ultraviolet ( UV), 320–380 nm/425 nm/435 nm; violet (V) 380–415 nm/455 nm/475 nm; blue (B) 450–490 nm/505 nm/515 nm; green (G) 495–555 nm/585 nm/595 nm |
| Application | laboratory/medical |
| Illumination position | double |
| Research method | bright field, phase contrast microscope, fluorescence |
| Set with pencil case/case/bag | dust cover |
