The MAGUS Lum V500L is an inverted microscope for fluorescence microscopy in reflected light and brightfield and phase contrast microscopy in transmitted light. For fluorescence microscopy, filters for DAPI, FITC and TRITC fluorochromes are used. The inverted design allows you to analyze samples contained in laboratory glassware, such as Petri dishes, test tubes, etc. The height of the glassware can reach 55 mm or 165 mm when the stand is tilted. The optics are designed for laboratory glassware with a 1.2 mm thick bottom. This microscope is ideal for research and routine laboratory work and can be used for teaching purposes.
Optics
Two options for viewing INSTALLATION OF A DIGITAL CAMERA AND MONITOR: The trinocular tube is designed to accommodate a monitor (not included), while the side port on the microscope frame is designed for a digital camera (not included). On both optical paths there is a beam splitter: 100/0 and 0/100 (microscope frame), 80/20 (trinocular head). The 45° inclination of the eyepiece tube allows you to make observations in complete comfort. When assembling the microscope, the user can rotate the optical tubes 180°.
The package includes six planachromatic objectives: three for use in bright field and fluorescence microscopy, the others three for phase contrast microscopy. The lenses are infinitely corrected and have a long working distance. The nosepiece revolver has six slots for housing as many objectives. The nosepiece is placed on a support underneath the stage.
Illumination
The fluorescence excitation source is a 5 W LED with a life of 50,000 hours, without the need for replacement during the useful life of the instrument. The instrument is equipped with three excitation filters: filters for DAPI, FITC and TRITC fluorochromes. LED lighting is more energy efficient and easier to maintain than a mercury vapor lamp, making it advisable for use in university-level education. LED lighting is consistent in terms of color temperature. The LED turns on and off quickly and does not overheat during prolonged use.
The light source for transmitted light observations is always a 5 W LED. Its light intensity is sufficient for each of the objectives used for bright field or phase contrast microscopy techniques.
Both light sources, both reflected and transmitted light, can be adjusted according to the Köhler method. This allows you to reach the maximum resolution limit for each objective lens and obtain a clear, sharp image without artefacts or edge darkening.
The microscope is equipped with a phase contrast condenser: three slots are used for phase contrast objectives with 10x, 20x and 40x magnification, while the fourth slot is for bright field microscopy. Phase contrast rings can be centered. You can switch from one microscopy technique to another just by turning a knob.
Stage and focusing mechanism
The fixed stage is equipped with a translator insert for moving laboratory glassware and is equipped with three supports for plates of various sizes. Laboratory glassware can be moved on the horizontal plane, along two axes perpendicular to each other. The microscope features a mechanism for coarse and fine focusing. The coarse focus mechanism is equipped with a fixing knob and the tension of the coarse screw can be adjusted. The knobs have an ergonomic design that allows the user to maintain a relaxed posture while working.
Accessories
The microscope can be equipped with additional eyepieces , calibration slides or a digital camera from the MAGUS line of accessories.
Key features:
- Inverted design for observation of contained samples in laboratory glassware with a bottom thickness of 1.2 mm and a height of up to 55 mm, or 165 mm with inclined support
- Microscopy techniques: fluorescence (reflected light), bright field and phase contrast (transmitted light)
- Köhler illumination configurable for transmitted and reflected light; light source with long-life 5 W LED, without the need to replace bulbs
- The instrument is equipped with three excitation filters: filters for DAPI, FITC and TRITC fluorochromes
- Phase contrast condenser, allows for quick and practical switching between the various microscopy techniques
- Trinocular head; two options for installing a camera or monitor: beam splitter with ratio 100/0 and 0/100 (microscope frame), 80/20 (trinocular head)
The package contains:
- Stand with power input, light source for observations in reflected and transmitted light, condenser, focusing mechanism, stage and nosepiece
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- Trinocular head
- Plan achromatic infinity objective: PLL 10x/0.25 for phase contrast, WD 4.3 mm
- Plan achromatic infinity objective: PLL 20х/0.40 WD 8.0 mm
- Plan achromatic infinity objective: PLL 40х/0.60 WD 3.5 mm
- Plan achromatic infinity objective: PLL 10x/ 0.25 PHP2 WD 4.3 mm
- Plan achromatic infinity objective: PLL 20x/0.40 PHP2 WD 8.0 mm
- Plan achromatic infinity objective: PLL 40x/ 0.60 PHP2 WD 3.5 mm
- 10x/22 mm eyepiece with long eye relief (2 pcs.)
- UV screen
- C-mount adapter 1x
- Allen key
- Power cable
- Dust cover
- User manual and warranty card
Available on request:
- 10x/22 mm eyepiece with graduated scale
- 12.5x/14 mm eyepiece (2 pcs .)
- Eyepiece 15x/15 mm (2 pcs.)
- Eyepiece 20x/12 mm (2 pcs.)
- Eyepiece 25x/9 mm (2 pcs.)
- Digital camera
- Calibration glass
| Brand | MAGUS |
| Warranty | 5 лет |
| EAN | 5905555018232 |
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| Package size (LxWxH) | 38x61x82 cm |
| Shipping weight | 24 kg |
| Type | biological, light/optical |
| Tested | trinocular |
| Vertical tube | Siedentopf, 180° rotatable |
| Magnification, x | 100–400 in basic configuration (*optional: 40–500/600/800/1000) |
| Eyepiece tube diameter, mm | 30 |
| Eyepieces | 10х/22 mm, eye relief: 10 mm (*optional: 10x/22 mm with graduated scale, 12.5x/14 ; 15x/15; 20x/12; 25x/9) |
| Objectives | plan achromatic objectives at infinity (∞): PLL 10x/0.25/4.3; PLL 20x/0.40/8.0; PLL 40x/0.60/3.5; phase contrast: PLL 10x/0.25/4.3 PHP2; PLL 20x/0.40/8.0 PHP2; PLL 40x/0.60/3.5 PHP2; parfocal distance: 45 mm |
| Nosepiece revolver | for 6 objectives |
| Working distance, mm | 4.3 (10х); 8.0 (20x); 3.5 (40x) |
| Interpupillary distance, mm | 48 — 75 |
| Stage, mm | 227x208 |
| Range of movement of the stage, mm | 77/134.5 |
| Table features | fixed, with translator insert and Ø118 mm glass plates; Ø118 mm; plate supports: 86x129.5 mm, Ø87.5 mm; 34x77.5 mm, Ø68.5 mm; 57x82 mm, Ø60 mm |
| Capacitor | NA 0.6; working distance: 55 mm; phase contrast turret; with fixing screws |
| Diaphragm | iris aperture diaphragm |
| Focus | coaxial knobs for coarse and fine focusing (macro and fine), positioned on both sides |
| Illumination | halogen, fluorescent |
| Brightness adjustment | yes |
| Power supply | AC mains, 85 –265 V, 50/60 Hz |
| Type of light source | light source for reflected light observations: 5 W LED; Light source for transmitted light observations: 5 W LED |
| Optical filters | yes |
| Operating temperature range, °C | 5 — 35 |
| Special features | phase contrast condenser (turret) with available housing and phase contrast ring plates for 10x, 20x, and 40x objectives; auxiliary centering microscope |
| User level | expert users, professionals |
| Level of difficulty assembly and installation | complicated |
| Fluorescent module | filters: ultraviolet (UV), violet (V), blue (B), green (G) |
| Fluorescence filter: filter type/excitation wavelength/dichroic mirror/emission wavelength | ultraviolet ( UV), 320–380 nm/425 nm/435 nm; violet (V) 380–415 nm/455 nm/475 nm; blue (B) 450–490 nm/505 nm/515 nm; green (G) 495–555 nm/585 nm/595 nm |
| Application | laboratory/medical |
| Illumination position | double |
| Research method | bright field, phase contrast microscope, fluorescence |
| Set with pencil case/case/bag | dust cover |
